4DZG

Crystal structure of Aeromonas hydrophila PliG, a periplasmic lysozyme inhibitor of g-type lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-26
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	76.259, 76.259, 62.443
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.317 - 2.020
R-factor	0.1854
Rwork	0.184
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.060	2.130
High resolution limit [Å]	2.020	2.020
Number of reflections	13867
<I/σ(I)>	16.6	2.5
Completeness [%]	98.1	92.3
Redundancy	8.9	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium cacodylate pH6.5, 1M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K