4DZB
Mucosal-associated invariant T cell receptor, Valpha7.2Jalpha33-Vbeta2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95453 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.962, 64.515, 155.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.19264 |
| Rwork | 0.191 |
| R-free | 0.23063 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nx5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.093 | 0.773 |
| Number of reflections | 44624 | |
| <I/σ(I)> | 13.7 | 2.5 |
| Completeness [%] | 97.1 | 92.6 |
| Redundancy | 7.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 25% PEG 1500, 0.1M MIB buffer (sodium malonate, imidazole, boric acid), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
