4DZ8

human IgG1 Fc fragment Heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.929, 81.292, 136.151
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.910
R-factor	0.20638
Rwork	0.205
R-free	0.23658
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.265
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	43830
<I/σ(I)>	28.7	3.44
Completeness [%]	99.9	100
Redundancy	6.9	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	50 mM Hepes, pH 7.0, 1% Tryptone, and 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K