4DY0
Crystal structure of native protease nexin-1 with heparin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.650, 140.650, 93.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.140 - 2.350 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.22634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.835 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0098) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.140 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.138 | |
| Number of reflections | 39468 | |
| <I/σ(I)> | 11.1 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | 25mM NaAc, 12% PEG3350, 0.2M (NH4)2SO4, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
