4DWW
Crystal Structure of Nattokinase from Bacillus subtilis natto
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.293, 49.899, 56.338 |
| Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
| Resolution | 41.369 - 1.740 |
| R-factor | 0.1366 |
| Rwork | 0.133 |
| R-free | 0.19870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1scj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.974 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.740 |
| Number of reflections | 14847 |
| Completeness [%] | 69.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 6.4mg/ml Nattokinase, 50mM Tris HCl, 50mM HEPES, 10% PEG 8000, 8% ethylene glycol, 5mM CaCl2, 100mM NaCl, 2mM PMSF, 4mM Pefabloc SC, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
