4DVX
Crystal structure of clade A/E 93TH057 HIV-1 gp120 core in complex with MAE-II-188
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-23 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.610, 68.295, 93.956 |
| Unit cell angles | 90.00, 91.47, 90.00 |
Refinement procedure
| Resolution | 28.461 - 2.400 |
| R-factor | 0.2035 |
| Rwork | 0.200 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.403 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 | 0.401 |
| Number of reflections | 26948 | |
| <I/σ(I)> | 16.34 | 1.7 |
| Completeness [%] | 83.6 | 27.9 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 5% iso-propanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
