4DVV
Crystal structure of clade A/E 93TH057 HIV-1 gp120 core in complex with AS-I-261
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-23 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.779, 68.079, 94.350 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 26.390 - 1.940 |
| R-factor | 0.1829 |
| Rwork | 0.181 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tgt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.390 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.058 | 0.330 |
| Number of reflections | 58230 | |
| <I/σ(I)> | 22 | 2.23 |
| Completeness [%] | 95.4 | 63.4 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10% PEG 8000, 5% iso-propanol, 0.1M HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.5 |
