4DV3
Crystal structure of the Thermus thermophilus 30S ribosomal subunit with a 16S rRNA mutation, C912A, bound with streptomycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 402.977, 402.977, 172.607 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.752 - 3.547 |
R-factor | 0.1689 |
Rwork | 0.166 |
R-free | 0.22020 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.598 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (dev_978) |
Refinement software | PHENIX (dev_978) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.680 |
High resolution limit [Å] | 3.546 | 7.610 | 3.546 |
Rmerge | 0.156 | 0.094 | 0.770 |
Number of reflections | 164584 | ||
<I/σ(I)> | 10.4 | ||
Completeness [%] | 96.6 | 93.5 | 90.9 |
Redundancy | 2.8 | 2.8 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |