Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.714, 135.058, 204.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.046 - 3.292 |
| R-factor | 0.2475 |
| Rwork | 0.245 |
| R-free | 0.30270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dvl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.7.3_928) |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.656 | 3.420 |
| High resolution limit [Å] | 3.292 | 3.292 |
| Rmerge | 0.099 | 0.426 |
| Number of reflections | 50551 | |
| <I/σ(I)> | 10.5 | 1.42 |
| Completeness [%] | 91.0 | 51.6 |
| Redundancy | 8.35 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 291 | sodium formate, glycerol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
