4DTT
Crystal structure of human insulin degrading enzyme (ide) in complex with compund 41367
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2010-10-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 |
| Unit cell lengths | 263.518, 263.518, 90.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.220 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cww |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.744 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 55194 | |
| Completeness [%] | 99.8 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 10-13% PEG MME 5000, 100 MM HEPES PH 7.0, 4-14% TACSIMATE, 10% DIOXANE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
