4DT1

Crystal structure of the Psy3-Csm2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGHT
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	135.123, 50.433, 76.833
Unit cell angles	90.00, 103.17, 90.00

Refinement procedure

Resolution	33.094 - 1.899
R-factor	0.1943
Rwork	0.192
R-free	0.22950
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.899	1.899
Number of reflections	38738
Completeness [%]	96.6	84.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	285	100mM Tris-HCl, 10% Glycerol, 14% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	8	285	100mM Tris-HCl, 10% Glycerol, 14% ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K