4DSR
Crystal structure of peroxiredoxin Ahp1 from Saccharomyces cerevisiae in reduced form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 |
| Unit cell lengths | 154.463, 154.463, 90.966 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.200 - 2.910 |
| R-factor | 0.24051 |
| Rwork | 0.239 |
| R-free | 0.27423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tp9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.401 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.010 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.131 | 0.369 |
| Number of reflections | 16465 | |
| <I/σ(I)> | 7.68 | 2.991 |
| Completeness [%] | 91.3 | 91.5 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% polyethylene glycol 3350, 0.2M ammonium sulfate, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
