4DSF
Ternary complex of Bacillus DNA Polymerase I Large Fragment F710Y, DNA duplex, and rCTP (paired with dG of template) in presence of Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Detector technology | CCD |
Collection date | 2011-01-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1159 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.680, 109.060, 150.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.063 - 1.661 |
R-factor | 0.1781 |
Rwork | 0.177 |
R-free | 0.19960 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4dse |
RMSD bond length | 0.011 |
RMSD bond angle | 1.379 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX (dev_1026) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.760 |
High resolution limit [Å] | 1.660 | 4.950 | 1.660 |
Rmerge | 0.059 | 0.035 | 0.491 |
Number of reflections | 164320 | ||
<I/σ(I)> | 19.33 | 45.1 | 3.62 |
Completeness [%] | 90.6 | 98.9 | 93.4 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 290 | 50% Saturated Ammonium Sulfate, 2.5% MPD, 10mM MnSO4, 100mM MES, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |