4DS7
Crystal structure of yeast calmodulin bound to the C-terminal fragment of spindle pole body protein Spc110
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97153 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.670, 145.444, 49.837 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.150 |
R-factor | 0.1992 |
Rwork | 0.197 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hr4 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.190 |
High resolution limit [Å] | 2.150 | 5.820 | 2.150 |
Rmerge | 0.070 | ||
Number of reflections | 47075 | ||
<I/σ(I)> | 31.184 | 80.894 | 3.876 |
Completeness [%] | 99.9 | 98.6 | 99.5 |
Redundancy | 15.9 | 15.7 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.5 | 293 | 0.1M sodium acetate, 0.7M ammonium sulfate, 0.5M guanidine chloride, 0.001M strontium chloride, 0.00005M copper chloride, pH 4.5, 0.0005M LAURYLDIMETHYLAMINE OXIDE, VAPOR DIFFUSION, temperature 293K |