4DS6

Crystal structure of a group II intron in the pre-catalytic state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-05
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	89.880, 95.310, 226.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.406 - 3.644
R-factor	0.205
Rwork	0.205
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3igi
RMSD bond length	0.008
RMSD bond angle	0.983
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.406
High resolution limit [Å]	3.644
Number of reflections	22036
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	303	0.5mM spermine, 10mM magnesium acetate, 50mM MES pH 5.6, 2.3M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 303K