4DR7
Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position, and streptomycin bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 402.490, 402.490, 174.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.909 - 3.750 |
R-factor | 0.1507 |
Rwork | 0.148 |
R-free | 0.20080 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.627 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (dev_978) |
Refinement software | PHENIX (dev_978) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.850 |
High resolution limit [Å] | 3.750 | 16.770 | 3.750 |
Rmerge | 0.136 | 0.014 | 0.014 |
Number of reflections | 146049 | 1750 | 10704 |
<I/σ(I)> | 15.52 | 59.28 | 1.99 |
Completeness [%] | 99.9 | 95 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 6.5 | 277 | MPD, pH 6.5, HANGING DROP, temperature 277K |