4DR5

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered cognate transfer RNA anticodon stem-loop and streptomycin bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 41 21 2
Unit cell lengths	400.510, 400.510, 175.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.902 - 3.450
R-factor	0.154
Rwork	0.152
R-free	0.19180
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (dev_978)
Refinement software	PHENIX (dev_978)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.540
High resolution limit [Å]	3.450	15.430	3.450
Rmerge	0.123	0.011	0.011
Number of reflections	184810	1204	13596
<I/σ(I)>	16.32	46.04	2.3
Completeness [%]	99.4	51.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	6.5	277	MPD, pH 6.5, HANGING DROP, temperature 277K