4DQZ

Crystal Structure of C-terminal Half of Bacterial Hen1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2011-10-08
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 65
Unit cell lengths	68.503, 68.503, 165.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.420 - 2.301
R-factor	0.2093
Rwork	0.204
R-free	0.25690
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_985))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	19215
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	15% PEG 400, 0.1M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K