4DQ7

Structural Investigation of Bacteriophage Phi6 Lysin (V207F mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Collection date	2011-01-01
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	62.120, 68.722, 89.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.010 - 1.400
R-factor	0.178
Rwork	0.175
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.001
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.420
High resolution limit [Å]	1.400	3.800	1.400
Rmerge	0.052	0.025
Number of reflections	73877
<I/σ(I)>	12.4
Completeness [%]	96.5	94.7	94.5
Redundancy	4.3	4.4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.6 M Na-Acetate, 0.1 M Na-Citrate, pH 6.5, vapor diffusion, hanging drop, temperature 289K