4DPL
Structure of malonyl-coenzyme A reductase from crenarchaeota in complex with NadP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.580, 128.800, 140.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1649 |
| Rwork | 0.163 |
| R-free | 0.19411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ys4 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.820 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 135044 | |
| <I/σ(I)> | 16.4 | 3.4 |
| Completeness [%] | 99.8 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 291 | 33% PEP 426, 0.1 M NaOAc, 0.15 M (NH4)2SO4, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
