4DOO

Crystal structure of Arabidopsis thaliana fatty-acid binding protein At3g63170 (AtFAP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-01
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	56.489, 56.901, 139.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 1.900
Rwork	0.200
R-free	0.24830
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.205
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.900
Number of reflections	36084

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1 M sodium TAPS, 19% (w/v) PEG 3350, 0.3 M potassium chloride, 2 mM dithiothreitol, pH 8.5, vapor diffusion, hanging drop, temperature 277K