4DOH
IL20/IL201/IL20R2 Ternary Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1. |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 103.115, 111.764, 136.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27800 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 38635 | |
| Completeness [%] | 90.7 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 19% PEG 6000, 0.1M ADA, 0.1M MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
