4DO2
Crystal Structure of the Rop protein mutant D30P/A31G at resolution 1.4 resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.817 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 26.669, 38.823, 56.644 |
Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
Resolution | 55.640 - 1.401 |
R-factor | 0.1602 |
Rwork | 0.159 |
R-free | 0.18770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rop |
RMSD bond length | 0.027 |
RMSD bond angle | 2.405 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.700 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 22394 | |
Completeness [%] | 99.5 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 45%(v/v) methanol, 50 mM HEPES pH 6.4 and 100 mM Li2SO4, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |