4DNW
Crystal structure of UVB-resistance protein UVR8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.07214 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.646, 61.517, 103.167 |
| Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
| Resolution | 32.294 - 1.773 |
| R-factor | 0.1726 |
| Rwork | 0.171 |
| R-free | 0.20120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dnu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 63040 | |
| Completeness [%] | 97.4 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.2 | 291 | 18%(w/v) PEG 8000, 100mM Tris pH9.2, 200mM Magnesium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
