4DNK
Crystal structure of a tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB) from homo sapiens at 2.20 A resolution.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91162,0.97946,0.97932 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.144, 122.848, 55.256 |
| Unit cell angles | 90.00, 113.98, 90.00 |
Refinement procedure
| Resolution | 29.227 - 2.200 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.22620 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.173 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.227 | 29.227 | 2.260 |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.043 | 0.753 | |
| Total number of observations | 1240 | 8349 | |
| Number of reflections | 31601 | ||
| <I/σ(I)> | 7.4 | 14.4 | 1 |
| Completeness [%] | 98.1 | 94.5 | 97.9 |
| Redundancy | 3.6 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20.00% polyethylene glycol 3350, 0.20M magnesium acetate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
