4DNE
Crystal structure of a triple-mutant of streptavidin in complex with desthiobiotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.720, 93.782, 104.413 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.860 - 1.880 |
| R-factor | 0.18323 |
| Rwork | 0.181 |
| R-free | 0.21897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mk5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.121 | 0.410 |
| Number of reflections | 19097 | |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mM MgSO4, 50 mM Na caodylate, 2M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
