Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 112.378, 112.378, 232.783 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.860 - 2.500 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1F3I |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.760 | 2.500 |
| Rmerge | 0.091 | 0.035 | 0.389 |
| Number of reflections | 31250 | ||
| <I/σ(I)> | 29.428 | 66.484 | 6.786 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 16.9 | 20 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 15% PEG 1500, 0.35M POTASSIUM GLUTAMATE, 0.05M POTASSIUM SUCCINATE, pH 5.0, microbatch, temperature 293K |
