4DLO

Crystal structure of the GAIN and HormR domains of brain angiogenesis inhibitor 3 (BAI3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	70
Detector technology	CCD
Collection date	2009-07-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	123.364, 128.008, 160.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.400 - 2.300
R-factor	0.1882
Rwork	0.186
R-free	0.22810
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.400	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Number of reflections	56485
Completeness [%]	99.7	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	15% PEG6000, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	15% PEG6000, 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K