4DL7
Human DNA polymerase eta fails to extend primer 2 nucleotide after cisplatin crosslink (Pt-GG4).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2010-10-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.000, 99.000, 81.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.970 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mr2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.815 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.158 | 0.650 |
| Number of reflections | 31415 | |
| <I/σ(I)> | 11.98 | 2.12 |
| Completeness [%] | 97.8 | 82.3 |
| Redundancy | 3.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | MES, MgCl2, PEG 2K-MME, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
