4DKK

The X-ray Crystal Structure of the Human STAU1 SSM-'RBD'5 Domain-Swapped Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97932
Spacegroup name	P 41 21 2
Unit cell lengths	45.741, 45.741, 86.086
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.346 - 1.701
R-factor	0.1668
Rwork	0.163
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	10665
<I/σ(I)>	28.9	10.6
Completeness [%]	99.8	99.7
Redundancy	13.8	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291.15	Drop:130 mM citric acid pH 2.5, 26% (w/v) PEG 6000 (final pH of 4), Reservoir:100 mM citric acid pH 2.5, 35% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K