4DK6

Structure of Editosome protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	77
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	173.026, 40.882, 61.281
Unit cell angles	90.00, 92.55, 90.00

Refinement procedure

Resolution	43.210 - 2.650
R-factor	0.226
Rwork	0.224
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k7u
RMSD bond length	0.005
RMSD bond angle	0.909
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.079	0.043	0.301
Number of reflections	12226
<I/σ(I)>	12.2
Completeness [%]	95.4	92.1	97.2
Redundancy	2.5	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M magnesium chloride, 0.1M bis-tris, 25% w/v PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K