4DIN
Novel Localization and Quaternary Structure of the PKA RI beta Holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 172.194, 172.194, 146.646 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.700 |
| Rwork | 0.211 |
| R-free | 0.29260 |
| Starting model (for MR) | 2qcs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.3 with DEN refinement) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.770 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.150 | 0.440 |
| Number of reflections | 16198 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 96.1 | 90 |
| Redundancy | 9.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 277 | 1.4 M ammonium sulfate, 0.1M Sodium Acetate pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
