4DHI

Structure of C. elegans OTUB1 bound to human UBC13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.034
Spacegroup name	P 61 2 2
Unit cell lengths	111.070, 111.070, 179.612
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.720 - 1.800
R-factor	0.18782
Rwork	0.187
R-free	0.20866
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1J7D and 2ZFY
RMSD bond length	0.012
RMSD bond angle	1.249
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.000
High resolution limit [Å]	1.800
Number of reflections	61151
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM sodium cacodylate, 1 M trisodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K