4DHE

Crystal structure of a probable GTP-binding protein engB from Burkholderia thailandensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97740
Spacegroup name	P 21 21 21
Unit cell lengths	48.940, 76.960, 134.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.781 - 2.200
R-factor	0.2368
Rwork	0.235
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pui
RMSD bond length	0.012
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.093	0.024	0.497
Number of reflections	26282	294	1906
<I/σ(I)>	13.31	42.94	2.99
Completeness [%]	99.0	83.5	98.8
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	ButhA.00252.a.A1.PW33397 at 34 mg/mL against JCSG+ H8, 0.2 M NaCl, 0.1 M BisTris, 25% PEG 3350 with 15% EG as cryo-protectant, Crystal tracking ID 225992h8, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K