4DFY
Crystal structure of R194A mutant of cAMP-dependent protein kinase with unphosphorylated activation loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.745, 95.745, 173.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.520 - 2.997 |
| R-factor | 0.25326 |
| Rwork | 0.252 |
| R-free | 0.28604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rdq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 2.997 | 2.997 |
| Number of reflections | 18017 | |
| <I/σ(I)> | 28.1 | 2.8 |
| Completeness [%] | 94.9 | 59.5 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 100 mM Tris, 800 mM sodium formate, 10% PEG 8000, 10% PEG 1000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
