4DDQ
Structural plasticity of the Bacillus subtilis GyrA homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 152.803, 165.106, 180.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.020 - 3.300 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ab4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS ((VERSION December 6) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_934)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.020 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.172 | 0.858 |
| Number of reflections | 69239 | |
| <I/σ(I)> | 7.37 | 1.06 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.3 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 295 | 0.1M Tris/HCl pH 9.0, 25% P400, 0.2M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
