4DDJ
Crystal structure of saposin A in complex with lauryldimethylamine-N-oxide (LDAO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 39.794, 39.794, 247.284 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.443 |
| Number of reflections | 9934 | |
| <I/σ(I)> | 44.9 | 3.2 |
| Completeness [%] | 98.7 | 98.1 |
| Redundancy | 9.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 300 | 1.6 M trisodium citrate, pH 6.5, VAPOR DIFFUSION, temperature 300K |
| 1 | VAPOR DIFFUSION | 6.5 | 300 | 1.6 M trisodium citrate, pH 6.5, VAPOR DIFFUSION, temperature 300K |
