4DCM

Crystal Structure of methyltransferase RlmG modifying G1835 of 23S rRNA in Escherichia coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-31
Detector	MAR CCD 165 mm
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	67.185, 42.134, 72.429
Unit cell angles	90.00, 104.46, 90.00

Refinement procedure

Resolution	29.984 - 2.297
R-factor	0.2033
Rwork	0.201
R-free	0.25210
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.297	2.300
Rmerge	0.060	0.530
Number of reflections	16968
<I/σ(I)>	39.71	5.62
Completeness [%]	95.2	100
Redundancy	7.2	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	0.2M Tris pH7.5, 5% (w/v) PEG 8000, 1% (w/v) protamine sulfate, 0.02M HEPES sodium pH6.8, VAPOR DIFFUSION, SITTING DROP, temperature 300K