4DCK

Crystal structure of the C-terminus of voltage-gated sodium channel in complex with FGF13 and CaM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 3
Unit cell lengths	126.041, 126.041, 126.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.858 - 2.200
R-factor	0.2107
Rwork	0.210
R-free	0.22690
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	0.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7.1_743)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.250
High resolution limit [Å]	2.200	5.780	2.210
Rmerge	0.067
Number of reflections	33622
<I/σ(I)>	39.3
Completeness [%]	99.8	99.5	94.8
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	20% PEG400, 150mM Magnesium Acetate, 50mM Sodium Cacodylate , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K
1	VAPOR DIFFUSION, SITTING DROP	6	289	20% PEG400, 150mM Magnesium Acetate, 50mM Sodium Cacodylate , pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K