4DC2
Structure of PKC in Complex with a Substrate Peptide from Par-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.456, 54.900, 82.489 |
| Unit cell angles | 90.00, 115.18, 90.00 |
Refinement procedure
| Resolution | 21.179 - 2.400 |
| R-factor | 0.1757 |
| Rwork | 0.172 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7_629)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.200 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 13400 |
| Completeness [%] | 82.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 3.5M sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
