4DBE

Crystal structure of orotidine 5'-monophosphate decarboxylase from Sulfolobus solfataricus complexed with inhibitor BMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 1 21 1
Unit cell lengths	72.414, 41.536, 73.117
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	32.751 - 1.789
R-factor	0.1973
Rwork	0.195
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dbd
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.751
High resolution limit [Å]	1.789
Number of reflections	41028
Completeness [%]	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG ME 550, 0.1M MES, 0.01M Zinc sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K