4DB9
Designed Armadillo repeat protein (YIIIM3AIII)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-08-09 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 131.920, 228.880, 116.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.730 - 2.400 |
| R-factor | 0.21282 |
| Rwork | 0.211 |
| R-free | 0.24579 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.730 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.107 |
| Number of reflections | 65534 |
| <I/σ(I)> | 5.2 |
| Completeness [%] | 100.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.03 | 293 | 1.55 M sodium malonate, pH 8.03, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
