4DAG

Structure of the Human Metapneumovirus Fusion Protein with Neutralizing Antibody Identifies a Pneumovirus Antigenic Site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	112
Detector technology	CCD
Collection date	2008-10-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 63 2 2
Unit cell lengths	258.960, 258.960, 167.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.853 - 3.390
R-factor	0.2613
Rwork	0.260
R-free	0.28950
Structure solution method	MIR
Starting model (for MR)	B20-4 Fab model PDB ENTRY 2FJH
RMSD bond length	0.011
RMSD bond angle	1.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	224.000
High resolution limit [Å]	3.200
Rmerge	0.179
Number of reflections	36420
Completeness [%]	72.7
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	16% PEG 5000 MME, 15% Glycerol, 100 mM Citrate pH 5.6. Crystals appeared after 7-15 days with two distinct morphologies: a square-like crystal and a longer, pointed crystal form., VAPOR DIFFUSION, HANGING DROP, temperature 293K