4D81
Metallosphera sedula Vps4 crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 65.269, 65.269, 112.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.520 - 2.400 |
| R-factor | 0.20971 |
| Rwork | 0.207 |
| R-free | 0.26283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.500 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.630 |
| Number of reflections | 12935 | |
| <I/σ(I)> | 16.7 | 2.3 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.8 | 40% (W/V) POLYETHYLENE GLYCOL 200 (PEG200) 100 MM TRIS-HCL BUFFER (PH 8.8) |
