4D7Y
Crystal structure of mouse C1QL1 globular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 68.080, 68.080, 65.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.040 - 1.440 |
| R-factor | 0.1415 |
| Rwork | 0.140 |
| R-free | 0.16930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c28 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.096 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.070 | 0.880 |
| Number of reflections | 20630 | |
| <I/σ(I)> | 16.9 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 0.02M NICKEL(II) CHLORIDE HEXAHYDRATE, 0.02M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.02M CADMIUM CHLORIDE HYDRATE, 0.1M SODIUM ACETATE TRIHYDRATE PH 4.5, 24% W/V POLYETHYLENE GLYCOL, MONOMETHYL ETHER 2,000 |
