4D7V
The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.308, 67.528, 65.592 |
| Unit cell angles | 90.00, 100.91, 90.00 |
Refinement procedure
| Resolution | 64.406 - 1.900 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.26720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAME PROTEIN WITH ONE METAL INSTEAD OF 3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.410 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.310 | 1.270 |
| Number of reflections | 29831 | |
| <I/σ(I)> | 6.8 | 2 |
| Completeness [%] | 99.3 | 98.4 |
| Redundancy | 6.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2M ZINC ACETATE, 17.5% PEG3350, pH 8 |
