4D7U
The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.258, 66.755, 66.077 |
| Unit cell angles | 90.00, 101.26, 90.00 |
Refinement procedure
| Resolution | 64.805 - 1.560 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.18760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eir |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.158 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.810 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.040 | |
| Number of reflections | 49150 | |
| <I/σ(I)> | 15.5 | 3.1 |
| Completeness [%] | 91.3 | 29.2 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 0.2M AMMONIUM CITRATE, 20% PEG 3350, PH 5.1 |
