4D7T
Structure of the SthK Carboxy-Terminal Region in complex with cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-03-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 4 |
| Unit cell lengths | 87.439, 87.439, 79.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.799 - 2.582 |
| R-factor | 0.2677 |
| Rwork | 0.265 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u11 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.014 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.050 | |
| Number of reflections | 18751 | |
| Completeness [%] | 99.1 | 96.6 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | PH 5 |
