4D7R
Crystal structure of a chimeric protein with the Sec7 domain of Rickettsia prowazekii RalF and the capping domain of Legionella pneumophila RalF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.690, 94.840, 47.140 |
| Unit cell angles | 90.00, 111.74, 90.00 |
Refinement procedure
| Resolution | 24.010 - 1.800 |
| R-factor | 0.1806 |
| Rwork | 0.178 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xsz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.790 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.670 |
| Number of reflections | 33357 | |
| <I/σ(I)> | 14.1 | 2.1 |
| Completeness [%] | 95.7 | 92.6 |
| Redundancy | 3.28 | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM CHLORIDE, 20% PEG 3350 |
