4D65
Structure of porin Omp-Pst2 from P. stuartii; the asymmetric unit contains a dimer of trimers.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.260, 135.510, 151.710 |
| Unit cell angles | 90.00, 114.61, 90.00 |
Refinement procedure
| Resolution | 45.718 - 2.200 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODELLER-MODEL OF OMP-PST2 BASED ON PDB ENTRY 4BD5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.540 |
| Number of reflections | 279377 | |
| <I/σ(I)> | 19.43 | 2 |
| Completeness [%] | 93.9 | 73.7 |
| Redundancy | 3.01 | 2.57 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 8% PEG 6000, 0.1 M TRIS PH 8, 1 M LICL |
